PacBio Sequencing: Principle, Steps, Types, Applications
PacBio sequencing, also known as Single Molecule Real-Time (SMRT) DNA Sequencing, is a type of third-generation sequencing method developed by Pacific Biosciences that allows the real-time observation...
View ArticleAmplicon Sequencing: Principle, Steps, Types, Applications
Amplicon sequencing is a method of sequencing that targets specific genomic regions or conserved regions within the organisms to characterize them and study genetic variations. Unlike whole-genome...
View ArticlePyrosequencing: Principle, Steps, Reactions, Types, Uses
Pyrosequencing is a sequencing method that determines the sequence of nucleotides by detecting the release of pyrophosphate (PPi) during nucleotide incorporation and monitors DNA synthesis in...
View ArticleOxford Nanopore Sequencing: Principle, Protocol, Uses
Oxford Nanopore Sequencing is a nanopore sequencing method developed by Oxford Nanopore Technologies (ONT) that identifies the sequence of nucleotides by passing individual molecules through nanoscale...
View ArticleWhole Transcriptome Sequencing (WTS) / Total RNA-Seq
Whole transcriptome sequencing (WTS), also known as total RNA sequencing (RNA-Seq) is a method of RNA sequencing that identifies all types of RNA molecules present in an organism or a ... Read more The...
View ArticleChIP Sequencing (ChIP-seq): Principle, Steps, Uses
ChIP Sequencing (ChIP-seq) is a method of sequencing that combines chromatin immunoprecipitation (ChIP) with sequencing to study DNA-protein interactions and the roles of DNA-binding proteins like...
View ArticleHigh Throughput Sequencing (HTS): Principle, Steps, Uses
High Throughput Sequencing (HTS) is a technology that allows the sequencing of multiple samples in a single run which increases the sequencing speed and efficiency compared to traditional sequencing...
View ArticleWhole Exome Sequencing (WES): Principle, Steps, Uses
Whole Exome Sequencing (WES) is a method of sequencing used to study the regions of the genome that code for proteins. These protein-coding regions within the genome are known as ... Read more The post...
View ArticleMethylation Sequencing: Principle, Methods, Steps, Uses
Methylation sequencing is a method of sequencing used to study DNA methylation patterns across the genome which is an important biological process that adds methyl group (CH3) to the cytosine ... Read...
View ArticleIon Torrent Sequencing: Principle, Steps, Method, Uses
Ion Torrent Sequencing is a next-generation sequencing method that uses a semiconductor chip to directly detect the hydrogen ions (H+) released during DNA synthesis. Ion Torrent technology introduced...
View ArticleSOLiD Sequencing: Principle, Steps, Applications
SOLiD (Sequencing by Oligonucleotide Ligation and Detection) is a second-generation sequencing platform that uses sequencing by ligation method to determine the sequence of nucleotides in target DNA....
View Article